A Preliminary Assessment of Silver Nanoparticle Inhibition of Monkeypox Virus Plaque Formation
نویسندگان
چکیده
The use of nanotechnology and nanomaterials in medical research is growing. Silver-containing nanoparticles have previously demonstrated antimicrobial efficacy against bacteria and viral particles. This preliminary study utilized an in vitro approach to evaluate the ability of silver-based nanoparticles to inhibit infectivity of the biological select agent, monkeypox virus (MPV). Nanoparticles (10–80 nm, with or without polysaccharide coating), or silver nitrate (AgNO3) at concentrations of 100, 50, 25, and 12.5 lg/mL were evaluated for efficacy using a plaque reduction assay. Both Ag-PS-25 (polysaccharide-coated, 25 nm) and Ag-NP-55 (non-coated, 55 nm) exhibited a significant (P B 0.05) dose-dependent effect of test compound concentration on the mean number of plaque-forming units (PFU). All concentrations of silver nitrate (except 100 lg/mL) and Ag-PS-10 promoted significant (P B 0.05) decreases in the number of observed PFU compared to untreated controls. Some nanoparticle treatments led to increased MPV PFU ranging from 1.04to 1.8-fold above controls. No cytotoxicity (Vero cell monolayer sloughing) was caused by any test compound, except 100 lg/mL AgNO3. These results demonstrate that silver-based nanoparticles of approximately 10 nm inhibit MPV infection in vitro, supporting their potential use as an anti-viral therapeutic.
منابع مشابه
Monkeypox virus.
INTRODUCTION ......... ..................................... I HISTORICAL NOTE ........... ................................... 2 Recognition of Monkeypox in Nonhuman Primates ....... ..................... 2 Human Infection with Monkeypox Virus ..................................... 3 CLASSIFICATION .......... .................................... 3 MONKEYPOX VIRUS AS AN INFECTIOUS AGENT ...... .....
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